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KMID : 0370220190630060367
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Yakhak Hoeji
2019 Volume.63 No. 6 p.367 ~ p.373
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Sustained Release of All-trans Retinoic Acid from Chitosan-coated Poly(DL-lactide-co-glycolide) Nanoparticles
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Chae Ji-Man
Oh In-Joon
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Abstract
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To investigate the effect of chitosan coating on the release of all-trans retinoic acid (ATRA) from poly(DLlactide-co-glycolide) (PLGA) nanoparticles, chitosan-coated nanoparticles were prepared with various concentrations ofchitosan and their characteristics were evaluated. Chitosan was easily coated on the surface of PLGA nanoparticles by asimple coating process. The particle size and zeta potential of the nanoparticles increased with increasing chitosan-coatingconcentrations. The results of transmission electron microscope (TEM) and Fourier transform-infrared spectroscopy (FT-IR)showed that chitosan was coated on the surface of the PLGA nanoparticles. The amount of chitosan adsorbed on thesurface of the PLGA nanoparticles, which was measured by the 2,4,6-trinitrobenzenesulfonic acid (TNBS) method,increased proportionally to the concentration of chitosan. The release of ATRA from the nanoparticles decreased withincreasing chitosan-coating and the initial burst effect was also greatly reduced. Pharmacokinetic parameters, such as halflifeand the mean residence time of ATRA drugs in the chitosan-coated nanoparticles, were significantly higher than thosein sodium ATRA solution or uncoated nanoparticles. In vitro cell experiments showed that the anticancer activity of ATRAin chitosan-coated PLGA nanoparticles was higher than that of free ATRA or uncoated nanoparticles. In conclusion,chitosan coating was shown to control the drug release by PLGA nanoparticles and chitosan-coated PLGA nanoparticlescan be considered suitable candidate carriers for sustained ATRA drug release.
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KEYWORD
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Poly(lactide-co-glycolide), PLGA nanoparticles, chitosan-coating, all-trans retinoic acid, drug release
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